dragonfly 600 laser confocal system (Oxford Instruments)

Oxford Instruments dragonfly 600 laser confocal system
Dragonfly 600 Laser Confocal System, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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axioplan microscope (Carl Zeiss)

Carl Zeiss axioplan microscope
Axioplan Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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axioplan microscope - by Bioz Stars, 2026-03

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mrc 600 laser scanning confocal microscope (Bio-Rad)

Bio-Rad mrc 600 laser scanning confocal microscope
Mrc 600 Laser Scanning Confocal Microscope, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mrc 600 laser scanning confocal microscope - by Bioz Stars, 2026-03

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mrc 600 confocal imaging instrument (Bio-Rad)

Bio-Rad mrc 600 confocal imaging instrument
Mrc 600 Confocal Imaging Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mrc 600 confocal imaging instrument - by Bioz Stars, 2026-03

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peak scanner (Thermo Fisher)

Thermo Fisher peak scanner
Peak Scanner, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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diaphot tmd inverted microscope (Nikon)

Nikon diaphot tmd inverted microscope
Diaphot Tmd Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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live cell imaging confocal microscope ultraview (Nikon)

Nikon live cell imaging confocal microscope ultraview
Live Cell Imaging Confocal Microscope Ultraview, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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axioplan 2 microscope (Carl Zeiss)

Carl Zeiss axioplan 2 microscope
Axioplan 2 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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microscope zeiss axioscope (Carl Zeiss)

Carl Zeiss microscope zeiss axioscope
Microscope Zeiss Axioscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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csux1 spinning disc confocal microscope (Nikon)

Nikon csux1 spinning disc confocal microscope
Csux1 Spinning Disc Confocal Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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amersham imager 600 (GE Healthcare)

GE Healthcare amersham imager 600

Amersham Imager 600, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gfp (Rockland Immunochemicals)

Rockland Immunochemicals gfp
$<t>CD63</t> negatively regulates mTORC1 signaling by Epstein-Barr virus LMP1. (A) LC-MS/MS spectral count quantification of LAMTOR proteins secreted in EVs from control and CD63 CRISPR cells. (B) HEK293 control or CD63 CRISPR cells were grown in serum-free medium and transfected with <t>GFP</t> or GFP-LMP1 for 24 h before cytoplasmic fractions were isolated, lysed, and analyzed by immunoblot analysis, with equal protein mass loaded. (C) Wild type (LMP1) and LMP1 C-terminal mutants containing the CTAR1 domain but lacking CTAR2 (CTAR1) or containing the CTAR2 domain but lacking CTAR1 (CTAR2) were induced by doxycycline in HK1 cells. Cell lysates were analyzed by immunoblotting for mTOR activation. All blots are representative images from 3 independent experiments. Quantitation of bands is based on averages from all experiments. (D) EVs were collected from HK1 cells following LMP1 mutant induction, and total vesicles were quantitated by nanoparticle tracking analysis. Un, uninduced. **, P < 0.01; *, P < 0.05.$
Gfp, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results

Journal: Molecular cell

Article Title: Transcription-mediated supercoiling regulates genome folding and loop formation

doi: 10.1016/j.molcel.2021.06.009

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: HRP-derived signal was detected with Pierce ECL Western Blotting Substrate kit (Thermo Fisher Scientific, #32106) on an Amersham Imager 600 (GE Healthcare Life Sciences, # 29083461).

Techniques: Recombinant, Modification, SYBR Green Assay, Imaging, Western Blot, Bicinchoninic Acid Protein Assay, Synthesized, Amplification, Labeling, Multiplex Assay, Plasmid Preparation, Sequencing, shRNA, Software, Microscopy, Confocal Microscopy

$CD63 negatively regulates mTORC1 signaling by Epstein-Barr virus LMP1. (A) LC-MS/MS spectral count quantification of LAMTOR proteins secreted in EVs from control and CD63 CRISPR cells. (B) HEK293 control or CD63 CRISPR cells were grown in serum-free medium and transfected with GFP or GFP-LMP1 for 24 h before cytoplasmic fractions were isolated, lysed, and analyzed by immunoblot analysis, with equal protein mass loaded. (C) Wild type (LMP1) and LMP1 C-terminal mutants containing the CTAR1 domain but lacking CTAR2 (CTAR1) or containing the CTAR2 domain but lacking CTAR1 (CTAR2) were induced by doxycycline in HK1 cells. Cell lysates were analyzed by immunoblotting for mTOR activation. All blots are representative images from 3 independent experiments. Quantitation of bands is based on averages from all experiments. (D) EVs were collected from HK1 cells following LMP1 mutant induction, and total vesicles were quantitated by nanoparticle tracking analysis. Un, uninduced. **, P < 0.01; *, P < 0.05.$

Journal: Journal of Virology

Article Title: Tetraspanin CD63 Bridges Autophagic and Endosomal Processes To Regulate Exosomal Secretion and Intracellular Signaling of Epstein-Barr Virus LMP1

doi: 10.1128/JVI.01969-17

Figure Lengend Snippet: CD63 negatively regulates mTORC1 signaling by Epstein-Barr virus LMP1. (A) LC-MS/MS spectral count quantification of LAMTOR proteins secreted in EVs from control and CD63 CRISPR cells. (B) HEK293 control or CD63 CRISPR cells were grown in serum-free medium and transfected with GFP or GFP-LMP1 for 24 h before cytoplasmic fractions were isolated, lysed, and analyzed by immunoblot analysis, with equal protein mass loaded. (C) Wild type (LMP1) and LMP1 C-terminal mutants containing the CTAR1 domain but lacking CTAR2 (CTAR1) or containing the CTAR2 domain but lacking CTAR1 (CTAR2) were induced by doxycycline in HK1 cells. Cell lysates were analyzed by immunoblotting for mTOR activation. All blots are representative images from 3 independent experiments. Quantitation of bands is based on averages from all experiments. (D) EVs were collected from HK1 cells following LMP1 mutant induction, and total vesicles were quantitated by nanoparticle tracking analysis. Un, uninduced. **, P < 0.01; *, P < 0.05.

Article Snippet: Blots were probed using the following antibodies: Alix (Q-19; Santa Cruz Biotechnology), HSC70 (B-6; Santa Cruz), TSG101 (C-2; Santa Cruz), CD63 (TS63; Abcam), LMP1 (CS1-4; Dako), phospho-IκBα (2859; Cell Signaling), GFP (600-101-215; Rockland), beta-actin (629630; GeneTex), phospho-mTOR Ser2481 (2974; Cell Signaling), phospho-mTOR Ser2448 (5536; Cell Signaling), mTOR (2983; Cell Signaling), Raptor (2280; Cell Signaling), Rictor (2114; Cell Signaling), GβL (3274; Cell Signaling), LAMTOR1 (8975; Cell Signaling), RagA (4357; Cell Signaling), RagB (8150; Cell Signaling), RagC (5466; Cell Signaling), RagD (4470; Cell Signaling), phospho-p70 S6 kinase Thr389 (9205; Cell Signaling), p70 S6 kinase (9202; Cell Signaling), LC3B (3868; Cell Signaling), Calnexin (H-70; Santa Cruz), CD81 (SC-9158; Santa Cruz), rabbit anti-mouse IgG (26728; GeneTex), rabbit anti-goat IgG (26741; GeneTex), and goat anti-rabbit IgG (Fab fragment) (27171; GeneTex).

Techniques: Liquid Chromatography with Mass Spectroscopy, CRISPR, Transfection, Isolation, Western Blot, Activation Assay, Quantitation Assay, Mutagenesis

Rescue of CD63 increases vesicle secretion of LMP1 and mTOR-associated proteins. GFP-LMP1 and fused CD63-BirA proteins were transfected into HEK293 control or CD63 CRISPR cells. Cell lysates and EVs were collected for immunoblot analysis of mTOR-associated protein levels 24 h after transfection. All blots are representative images from independent experiments.

Journal: Journal of Virology

Article Title: Tetraspanin CD63 Bridges Autophagic and Endosomal Processes To Regulate Exosomal Secretion and Intracellular Signaling of Epstein-Barr Virus LMP1

doi: 10.1128/JVI.01969-17

Figure Lengend Snippet: Rescue of CD63 increases vesicle secretion of LMP1 and mTOR-associated proteins. GFP-LMP1 and fused CD63-BirA proteins were transfected into HEK293 control or CD63 CRISPR cells. Cell lysates and EVs were collected for immunoblot analysis of mTOR-associated protein levels 24 h after transfection. All blots are representative images from independent experiments.

Techniques: Transfection, CRISPR, Western Blot

CD63 CRISPR cells develop large nonlysosomal cellular compartments. (A) HEK293 control or CD63 CRISPR cells were transfected with GFP to visualize large cellular structures by confocal microscopy. Hoechst stain was used to identify nuclear compartments. (B) Orthogonal projection of a representative CD63 CRISPR cell. (C) Cells were stained by LysoTracker to examine acidified compartments within cells. (D) Quantification of total LysoTracker-positive compartments in cells (n = 20 cells). Size bars, 20 μm.

Journal: Journal of Virology

Article Title: Tetraspanin CD63 Bridges Autophagic and Endosomal Processes To Regulate Exosomal Secretion and Intracellular Signaling of Epstein-Barr Virus LMP1

doi: 10.1128/JVI.01969-17

Figure Lengend Snippet: CD63 CRISPR cells develop large nonlysosomal cellular compartments. (A) HEK293 control or CD63 CRISPR cells were transfected with GFP to visualize large cellular structures by confocal microscopy. Hoechst stain was used to identify nuclear compartments. (B) Orthogonal projection of a representative CD63 CRISPR cell. (C) Cells were stained by LysoTracker to examine acidified compartments within cells. (D) Quantification of total LysoTracker-positive compartments in cells (n = 20 cells). Size bars, 20 μm.

Techniques: CRISPR, Transfection, Confocal Microscopy, Staining

CD63 knockout induces the development of large autophagic vacuoles. HEK293 control (A and B) or CD63 CRISPR (C and D) cells were fixed and stained for transmission electron microscopy to visualize large intracellular vacuoles. Black arrows denote vacuoles, and white arrows denote MVBs. Size bar, 1 μm. HEK293 control (E) or CD63 CRISPR (F) cells were transfected with GFP following serum starvation for 24 h. (G) Cells were treated with chloroquine or wortmannin under serum-deprived conditions for 2 h. LC3 levels were measured by immunoblot analysis. Blots are representative of three independent experiments. (H) Cells were transfected with GFP and stained with mono-dansylcadaverine (MDC) to examine the morphology and localization of autophagic vacuoles. (I) Orthogonal sections of CD63 CRISPR cells stained with MDC. (J) HEK293 CD63 CRISPR cells were transfected with GFP-LC3 and grown under full-serum or serum-free conditions before staining with MDC to visualize vacuoles by confocal microscopy. (K) Nasopharyngeal carcinoma cells (HK1) and an HK1 CD63 CRISPR derivative cell line were transfected with GFP and stained with MDC to visualize autophagic vacuoles. Size bars, 20 μm.

Journal: Journal of Virology

Article Title: Tetraspanin CD63 Bridges Autophagic and Endosomal Processes To Regulate Exosomal Secretion and Intracellular Signaling of Epstein-Barr Virus LMP1

doi: 10.1128/JVI.01969-17

Figure Lengend Snippet: CD63 knockout induces the development of large autophagic vacuoles. HEK293 control (A and B) or CD63 CRISPR (C and D) cells were fixed and stained for transmission electron microscopy to visualize large intracellular vacuoles. Black arrows denote vacuoles, and white arrows denote MVBs. Size bar, 1 μm. HEK293 control (E) or CD63 CRISPR (F) cells were transfected with GFP following serum starvation for 24 h. (G) Cells were treated with chloroquine or wortmannin under serum-deprived conditions for 2 h. LC3 levels were measured by immunoblot analysis. Blots are representative of three independent experiments. (H) Cells were transfected with GFP and stained with mono-dansylcadaverine (MDC) to examine the morphology and localization of autophagic vacuoles. (I) Orthogonal sections of CD63 CRISPR cells stained with MDC. (J) HEK293 CD63 CRISPR cells were transfected with GFP-LC3 and grown under full-serum or serum-free conditions before staining with MDC to visualize vacuoles by confocal microscopy. (K) Nasopharyngeal carcinoma cells (HK1) and an HK1 CD63 CRISPR derivative cell line were transfected with GFP and stained with MDC to visualize autophagic vacuoles. Size bars, 20 μm.

Techniques: Knock-Out, CRISPR, Staining, Transmission Assay, Electron Microscopy, Transfection, Western Blot, Confocal Microscopy

$Extracellular vesicle secretion and autophagic processes regulate LMP1-mediated intracellular signaling. Confocal microscopy of GFP-LMP1-transfected control or CD63 CRISPR cells following chloroquine (A) and wortmannin (B) treatment. Vacuoles were stained with MDC before live-cell imaging. Size bars, 20 μm. N, nuclear compartments. (C) Immunoblot analysis of EVs derived from HEK293 control or CD63 CRISPR cells following transfection of GFP-LMP1 and treatment with chloroquine or wortmannin for 24 h, with equal volumes loaded. (D) Immunoblots of corresponding cell lysates, with equal protein mass loaded. Nanoparticle tracking analysis of the quantity (E) and size (F) of EVs from cells following treatment with autophagy inhibitors. (G) Immunoblot analysis of cytoplasmic cellular fractions of cells transfected with GFP-LMP1, with equal protein mass loaded. Blots are representative images from repeated independent experiments. ***, P < 0.001; *, P < 0.05.$

Journal: Journal of Virology

Article Title: Tetraspanin CD63 Bridges Autophagic and Endosomal Processes To Regulate Exosomal Secretion and Intracellular Signaling of Epstein-Barr Virus LMP1

doi: 10.1128/JVI.01969-17

Figure Lengend Snippet: Extracellular vesicle secretion and autophagic processes regulate LMP1-mediated intracellular signaling. Confocal microscopy of GFP-LMP1-transfected control or CD63 CRISPR cells following chloroquine (A) and wortmannin (B) treatment. Vacuoles were stained with MDC before live-cell imaging. Size bars, 20 μm. N, nuclear compartments. (C) Immunoblot analysis of EVs derived from HEK293 control or CD63 CRISPR cells following transfection of GFP-LMP1 and treatment with chloroquine or wortmannin for 24 h, with equal volumes loaded. (D) Immunoblots of corresponding cell lysates, with equal protein mass loaded. Nanoparticle tracking analysis of the quantity (E) and size (F) of EVs from cells following treatment with autophagy inhibitors. (G) Immunoblot analysis of cytoplasmic cellular fractions of cells transfected with GFP-LMP1, with equal protein mass loaded. Blots are representative images from repeated independent experiments. ***, P < 0.001; *, P < 0.05.

Techniques: Confocal Microscopy, Transfection, CRISPR, Staining, Live Cell Imaging, Western Blot, Derivative Assay

Autophagy activation by trehalose drives CD63-independent LMP1 release. (A) Confocal microscopy of GFP-LMP1-transfected control or CD63 CRISPR cells following trehalose treatment. Vacuoles were stained with MDC before live-cell imaging. Size bars, 20 μm. N, nuclear compartments. (B) Immunoblot analysis of cytoplasmic lysates from HEK293 control or CD63 CRISPR cells following transfection of GFP or GFP-LMP1 and treatment with trehalose for 24 h, with equal protein mass loaded. (C) Immunoblots of corresponding cell-derived EVs, with equal volumes loaded. Blots are representative images from repeated independent experiments. Quantitation of LMP1 secreted following trehalose treatment was normalized to parental cell secretion over multiple experiments.

Journal: Journal of Virology

Article Title: Tetraspanin CD63 Bridges Autophagic and Endosomal Processes To Regulate Exosomal Secretion and Intracellular Signaling of Epstein-Barr Virus LMP1

doi: 10.1128/JVI.01969-17

Figure Lengend Snippet: Autophagy activation by trehalose drives CD63-independent LMP1 release. (A) Confocal microscopy of GFP-LMP1-transfected control or CD63 CRISPR cells following trehalose treatment. Vacuoles were stained with MDC before live-cell imaging. Size bars, 20 μm. N, nuclear compartments. (B) Immunoblot analysis of cytoplasmic lysates from HEK293 control or CD63 CRISPR cells following transfection of GFP or GFP-LMP1 and treatment with trehalose for 24 h, with equal protein mass loaded. (C) Immunoblots of corresponding cell-derived EVs, with equal volumes loaded. Blots are representative images from repeated independent experiments. Quantitation of LMP1 secreted following trehalose treatment was normalized to parental cell secretion over multiple experiments.

Techniques: Activation Assay, Confocal Microscopy, Transfection, CRISPR, Staining, Live Cell Imaging, Western Blot, Derivative Assay, Quantitation Assay

LMP1 promotes acidification of CD63 knockout-induced autophagic vacuoles. HEK293 control and CD63 CRISPR cells were transfected with GFP-LMP1 and stained with Hoechst (A) and MDC (B). (C) MDC-positive and total vacuoles were quantified for each cell (n = 30 cells). (D) Proportion of MDC-positive over total vacuoles per CD63 CRISPR cell. (E) HK1 control and CD63 CRISPR cells were transfected with GFP to visualize vacuoles. EBV-infected HK1 (HK1 + EBV) control cells and cells containing CD63 CRISPR were treated similarly to visualize autophagic vacuoles. (F) Cells were transfected with GFP-LMP1 and stained with LysoTracker. Arrowheads represent colocalization of LMP1 with lysosomal compartments. (G) LysoTracker-positive compartments quantitated for each cell (n = 20 cells). N, nuclear compartments. *, P < 0.05.

Journal: Journal of Virology

Article Title: Tetraspanin CD63 Bridges Autophagic and Endosomal Processes To Regulate Exosomal Secretion and Intracellular Signaling of Epstein-Barr Virus LMP1

doi: 10.1128/JVI.01969-17

Figure Lengend Snippet: LMP1 promotes acidification of CD63 knockout-induced autophagic vacuoles. HEK293 control and CD63 CRISPR cells were transfected with GFP-LMP1 and stained with Hoechst (A) and MDC (B). (C) MDC-positive and total vacuoles were quantified for each cell (n = 30 cells). (D) Proportion of MDC-positive over total vacuoles per CD63 CRISPR cell. (E) HK1 control and CD63 CRISPR cells were transfected with GFP to visualize vacuoles. EBV-infected HK1 (HK1 + EBV) control cells and cells containing CD63 CRISPR were treated similarly to visualize autophagic vacuoles. (F) Cells were transfected with GFP-LMP1 and stained with LysoTracker. Arrowheads represent colocalization of LMP1 with lysosomal compartments. (G) LysoTracker-positive compartments quantitated for each cell (n = 20 cells). N, nuclear compartments. *, P < 0.05.

Techniques: Knock-Out, CRISPR, Transfection, Staining, Infection

mrc 600 confocal imaging instrument Search Results

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